CH 633 Seminar: Arpa Ebrahimi
Proteomic studies analyze large protein libraries to aid in identifying biological markers for diagnosis, antibody and vaccine production, and drug targeting within pathogenic studies. Single-cell proteomics promises to reveal the full complexity of cellular diversity by enabling the analysis of biological processes at a single-cell level. However, a challenge in single-cell proteomics is the tedious and time-consuming sample preparation workflow, the need for optimized nanoflow separation, and ultrasensitive mass spectrometry. Herein, we present a single-cell proteomics
workflow based upon nanoliter additions of all the reagents using a microfluidic dispenser. Single-cell proteomic profiles were acquired using a Waters nanoflow UPLC system coupled to an Orbitrap Fusion Lumos mass spectrometer. The developed single-cell proteomic workflow using a microfluidic digital dispenser is robust and promises to broadly apply to the analysis of other mammalian cells. We envision that single-cell proteomics has the potential to transform biomedical research by providing access to cell-type specific proteome information at the single-cell level, for instance, in needle biopsy samples, to advance patient stratification and personalized medicine.
Thursday, December 1 at 4:00pm
Linus Pauling Science Center, 402
2900 SW Campus Way, Corvallis, OR 97331