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Development of a Single-Cell Proteomic Platform to Study HEK-293 Cells

CH 633 Seminar: Arpa Ebrahimi

Proteomic studies analyze large protein libraries to aid in identifying biological markers for diagnosis, antibody and vaccine production, and drug targeting within pathogenic studies. Single-cell proteomics promises to reveal the full complexity of cellular diversity by enabling the analysis of biological processes at a single-cell level. However, a challenge in single-cell proteomics is the tedious and time-consuming sample preparation workflow, the need for optimized nanoflow separation, and ultrasensitive mass spectrometry. Herein, we present a single-cell proteomics
workflow based upon nanoliter additions of all the reagents using a microfluidic dispenser. Single-cell proteomic profiles were acquired using a Waters nanoflow UPLC system coupled to an Orbitrap Fusion Lumos mass spectrometer. The developed single-cell proteomic workflow using a microfluidic digital dispenser is robust and promises to broadly apply to the analysis of other mammalian cells. We envision that single-cell proteomics has the potential to transform biomedical research by providing access to cell-type specific proteome information at the single-cell level, for instance, in needle biopsy samples, to advance patient stratification and personalized medicine.

Thursday, December 1 at 4:00pm

Linus Pauling Science Center, 402
2900 SW Campus Way, Corvallis, OR 97331

Event Type

Lecture or Presentation

Event Topic

Research

Audience

Faculty and Staff, Student, Alumni, Industry Partner, Online

County

Benton

Tags

Seminar

Organization
College of Science, Department of Chemistry
Contact Name

Luanne Johnson

Contact Email

luanne.johnson@oregonstate.edu

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